E sequences for making GFP fusion constructs were 12?7 h before microscopic observation. Transfected HEK293a cells derived from barley (Hordeum vulgare) proaleurain (Jiang and were incubated for 12?7 h before fixation using 4 paraformaldeRogers, 1998) or native signal peptide sequences of EMP homohyde. The immunolabeling process for HEK293a cells was as previlogues according to SignalP 3.0 Server
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